| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.251551 |
| Chromosome: | chromosome 16 |
| Location: | 1060175 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649466 | CAX5 | (1 of 3) K07300 - Ca2+:H+ antiporter (chaA, CAX); CAX family cation antiporter, membrane protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGTGTCCGCCTGTTGTTGCTGCTGCTG |
| Internal bar code: | GGGGAAATAAAACGATGGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 96 |
| LEAP-Seq percent confirming: | 80.4196 |
| LEAP-Seq n confirming: | 115 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGCCCTAGTGTTGGGTG |
| Suggested primer 2: | CGGTAACACGCATCTCACAC |