| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251564 |
| Chromosome: | chromosome 2 |
| Location: | 4326574 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099200 | (1 of 1) K06100 - symplekin (SYMPK) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGTGCGCAAGCAGCCAAGCGCGCAAGTC |
| Internal bar code: | GCCCGCAACCGTATAGTAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 466 |
| LEAP-Seq percent confirming: | 97.8749 |
| LEAP-Seq n confirming: | 829 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACGGTACATCTCCACTT |
| Suggested primer 2: | CAGCAAGGGACGAATGTACC |