| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.251579 |
| Chromosome: | chromosome 16 |
| Location: | 6549504 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672500 | (1 of 1) PTHR28573//PTHR28573:SF1 - FAMILY NOT NAMED // SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCTATCCACCTGCACCTCTACCGCTCC |
| Internal bar code: | TGCGGCGACCCGCGTTACACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 365 |
| LEAP-Seq percent confirming: | 97.9167 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATTGCTAGTTGCCAGAC |
| Suggested primer 2: | TAACCGCAGGTCATCTAGGG |