| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251614 |
| Chromosome: | chromosome 8 |
| Location: | 3367864 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374250 | SAG1 | (1 of 80) IPR003882 - Pistil-specific extensin-like protein; Plus agglutinin protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGAGGCATGGCCGACCGACCAACCTTGG |
| Internal bar code: | CCCATGAAATCCGTTGGCCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 99.1247 |
| LEAP-Seq n confirming: | 453 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGGTCGTCTAAGGCAG |
| Suggested primer 2: | CCTGGCTACAACTCGCTTCT |