| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251616 |
| Chromosome: | chromosome 2 |
| Location: | 5513329 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108350 | (1 of 24) PTHR10641 - MYB-LIKE DNA-BINDING PROTEIN MYB | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGTTTGAGAGCCGACTGATTGTCACTG |
| Internal bar code: | TTCAGTGATGAAGGGCTCTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 892 |
| LEAP-Seq percent confirming: | 98.6425 |
| LEAP-Seq n confirming: | 10972 |
| LEAP-Seq n nonconfirming: | 151 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCTACGGGTTCGTTGAG |
| Suggested primer 2: | ATAGATGTCGCTCAAACCCG |