| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251635 |
| Chromosome: | chromosome 17 |
| Location: | 3372674 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723700 | (1 of 2) PTHR32246:SF18 - C2 DOMAIN-CONTAINING PROTEIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACTCTTGTGCGTCTTCGTCTTGCACTTC |
| Internal bar code: | GGTGGATTTTGTGACTCGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 972 |
| LEAP-Seq percent confirming: | 98.388 |
| LEAP-Seq n confirming: | 1709 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCCTTGTTTGGGTTGAC |
| Suggested primer 2: | TGCCTGTCTGCAATCTTTTG |