| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251660 |
| Chromosome: | chromosome 13 |
| Location: | 2132107 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g577900 | (1 of 1) PF13865 - C-terminal duplication domain of Friend of PRMT1 (FoP_duplication) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAGCCCTCCAGCTCGGCATCGAGCTCCG |
| Internal bar code: | GAGGATGGTGCACCCCAACGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 239 |
| LEAP-Seq percent confirming: | 95.1351 |
| LEAP-Seq n confirming: | 176 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTGGATTCAAGGAAGGG |
| Suggested primer 2: | TACTTGGTGGTCGTGTTGGA |