| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251668 |
| Chromosome: | chromosome 16 |
| Location: | 6510173 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672850 | (1 of 1) IPR005018//IPR006593 - DOMON domain // Cytochrome b561/ferric reductase transmembrane; Conserved hypothetical membrane protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCGCCTTCCGGCCTCCACGCACGCCCT |
| Internal bar code: | CGATCCCGCCCCGAAAAACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 545 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCACAAGCGAACATAGCA |
| Suggested primer 2: | TGTGACCGACACCAATGAGT |