Insertion junction: LMJ.RY0402.251672_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.251672
Chromosome:chromosome 17
Location:2755166
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g718450 MMP2 Matrix metalloproteinase; (1 of 1) IPR003108 - Growth-arrest-specific protein 2 domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCGTGGGCCGCACCCACGGCCGCACCCG
Internal bar code:CCCCGCGGGGTCATGCGTAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:79
LEAP-Seq percent confirming:75.7143
LEAP-Seq n confirming:53
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGGCACATCACAATAAGG
Suggested primer 2:GGCGAGCACATGGAGAATAC