Insertion junction: LMJ.RY0402.251679_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.251679
Chromosome:chromosome 16
Location:1358202
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g651900 CRI1,OPR129,OPR126,CRTISO OctotricoPeptide Repeat protein 129 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGTGTGGGGTGGTGGCGCTGCTGGCGGG
Internal bar code:GGATTAGCTTTCTTTGCAATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:636
LEAP-Seq percent confirming:99.5061
LEAP-Seq n confirming:8260
LEAP-Seq n nonconfirming:41
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TACACACCACCCACCGTATG
Suggested primer 2:CCGTAGATCCATAGCCGTGT