| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251692 |
| Chromosome: | chromosome 7 |
| Location: | 1207061 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321100 | (1 of 1) PTHR10196:SF67 - SEDOHEPTULOKINASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTATCGTGTAATGTGCACGTTGCGTCGCA |
| Internal bar code: | ATGCGGCCGCGGATATACTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 635 |
| LEAP-Seq percent confirming: | 98.411 |
| LEAP-Seq n confirming: | 929 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCACCTTCAAAGCACAC |
| Suggested primer 2: | AGGTTTGGATAAGTGGCACG |