Insertion junction: LMJ.RY0402.251728_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.251728
Chromosome:chromosome_8
Location:4145127
Confidence (%):95
Locus disrupted Locus common name Defline Orientation Feature
Cre08.g379900 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TTGAAGTGGAGGAGGCAATGCAGCGCCGCG
Internal bar code:AGGCTCAGACCATACCGGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:415
LEAP-Seq percent confirming:99.7
LEAP-Seq n confirming:997
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGTGTGACAAGGGGAAG
Suggested primer 2:ACAGATGAACACCACCACGA