| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.251737 |
| Chromosome: | chromosome 7 |
| Location: | 2724785 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331150 | (1 of 2) 1.3.3.5 - Bilirubin oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACTTCTCTGGGCGGGCCATCTCCTCGAG |
| Internal bar code: | CTTTTATGCTCACTGACGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 237 |
| LEAP-Seq percent confirming: | 98.5714 |
| LEAP-Seq n confirming: | 207 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGAACAATTTTGTGGTC |
| Suggested primer 2: | GGCAAGATAGAGGCTTGACG |