| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251746 |
| Chromosome: | chromosome 5 |
| Location: | 3013092 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATACCGTACATCAATGCTCACAACACAGC |
| Internal bar code: | CTGCGGGAATACTGGGGAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 127 |
| LEAP-Seq percent confirming: | 45.8333 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 78 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGTCATACCAGTCGCAGA |
| Suggested primer 2: | GGACACAAAGCCAATTTCGT |