| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251749 |
| Chromosome: | chromosome 12 |
| Location: | 1481844 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487601 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCAACTGAGTGTGCGGCTCCAGCCACCA |
| Internal bar code: | TGGGTTCCCTCTTTCCTTTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 40.625 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGGAATAGGCAGCTTGG |
| Suggested primer 2: | GCAGAGTCGCGTTAAAGGTC |