| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.251751 |
| Chromosome: | chromosome 10 |
| Location: | 2943215 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440450 | PSB28 | (1 of 1) K08903 - photosystem II 13kDa protein (psb28); Photosystem II associated subunit 28 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTAAATGCACGTGCACGGTAGCCCCCCA |
| Internal bar code: | ATGTTTATTCTAACCTTTCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 380 |
| LEAP-Seq percent confirming: | 96.0272 |
| LEAP-Seq n confirming: | 8460 |
| LEAP-Seq n nonconfirming: | 350 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAAATGGTAGAGGCGATG |
| Suggested primer 2: | TTGCAAGAAGTTGTGGCTTG |