| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.251773 |
| Chromosome: | chromosome 17 |
| Location: | 5864628 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g739752 | FTSHi1,CTAP1,FTSHI1 | (1 of 3) IPR000642//IPR003593//IPR003959//IPR027417 - Peptidase M41 // AAA+ ATPase domain // ATPase, AAA-type, core // P-loop containing nucleoside triphosphate hydrolase; Chloroplast-import FtsH-like ATPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACACCGGCGCTGCTCTGCTTGCTAGCAC |
| Internal bar code: | GAGGGACTCGACGTACGTACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 99.1171 |
| LEAP-Seq n confirming: | 4266 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTCCAGTCCTGATGTGA |
| Suggested primer 2: | ACTCACTACACAGGTGGCCC |