Insertion junction: LMJ.RY0402.251784_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.251784
Chromosome:chromosome 8
Location:2522105
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCATGTCAAGCCTCAGCGAGCTCCCCGC
Internal bar code:GATACGTGCCTGATACTCCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:570
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACACCAGACCAGACCAGACC
Suggested primer 2:AGCGGTCTCCTTCATCTTGA