| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.251824 |
| Chromosome: | chromosome_5 |
| Location: | 3126849 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g239200 | FAP275 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCAAGTCGAAAGCAACACGTGGTTAGGGGA |
| Internal bar code: | TTCAGCAAAGTACGACACATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1129 |
| LEAP-Seq percent confirming: | 99.219 |
| LEAP-Seq n confirming: | 2922 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTTTACCGAGGACGTGT |
| Suggested primer 2: | CCGAAGTCTGAATCACAGCA |