Insertion junction: LMJ.RY0402.251831_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.251831
Chromosome:chromosome 7
Location:4166674
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g342450 SEE3 Putative splicing endonuclease positive effector; (1 of 3) K10706 - senataxin [EC:3.6.4.-] (SETX, ALS4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCCACGGAAGCTGGCGGCGGCGACTACGG
Internal bar code:TGAATGCTTGCAGCACATGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:409
LEAP-Seq percent confirming:55.3571
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GACGAGGATGAGGATGAGGA
Suggested primer 2:CAACTGCAAAACCACCAGAA