| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251845 |
| Chromosome: | chromosome 2 |
| Location: | 1482174 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCATGCTGTCAGCGCCTCAGCAGGGTTC |
| Internal bar code: | TACCCGGCCCAGCGACGAAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 655 |
| LEAP-Seq percent confirming: | 98.4029 |
| LEAP-Seq n confirming: | 4621 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGGGGGTACTAGGAGTC |
| Suggested primer 2: | GGCTACTTCCTGTCTGCTGC |