| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.251845 |
| Chromosome: | chromosome 14 |
| Location: | 1432530 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617750 | PDE1,PDE3 | (1 of 4) K13293 - cAMP-specific phosphodiesterase 4 (PDE4); 3'%252C5'-cyclic-nucleotide phosphodiesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTATACTGCCGCGCGCGCAGGTACAAGC |
| Internal bar code: | TCCCCACTTCTTACGTTATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 813 |
| LEAP-Seq percent confirming: | 99.3939 |
| LEAP-Seq n confirming: | 3280 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGCGTACGTGTTGAAAT |
| Suggested primer 2: | TCCTGTTCTTGCAGTTGTCG |