Insertion junction: LMJ.RY0402.251858_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.251858
Chromosome:chromosome_9
Location:4151834
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g393954 FAP106 Flagellar Associated Protein sense 5'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):AACTTATTCTTGCCTCAGTACCTTACACGC
Internal bar code:ACTCCAGTTTGTGATAGCCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:610
LEAP-Seq percent confirming:97.2912
LEAP-Seq n confirming:862
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:TGATCGAGTGTGTCGCTTTC
Suggested primer 2:CATTGGCACTTTCCCGTAGT