| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251863 |
| Chromosome: | chromosome 17 |
| Location: | 4251456 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730550 | (1 of 3) PTHR22298:SF39 - ENDOGLUCANASE 7 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATTCGTACGGTTCCCATCACAGTCCATG |
| Internal bar code: | CCACCGGACGTGCCTTTTTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1114 |
| LEAP-Seq percent confirming: | 99.4399 |
| LEAP-Seq n confirming: | 5326 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATATCCAGGAAGGTGCCG |
| Suggested primer 2: | CTTCTCAGGAGCTTTGTGGG |