| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251863 |
| Chromosome: | chromosome 17 |
| Location: | 4251467 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730550 | (1 of 3) PTHR22298:SF39 - ENDOGLUCANASE 7 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGGACACGCATGCTGACGTGGCTGTTGT |
| Internal bar code: | GTCGGTGAAGGATTATGGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 839 |
| LEAP-Seq percent confirming: | 98.8229 |
| LEAP-Seq n confirming: | 1763 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATATCCAGGAAGGTGCCG |
| Suggested primer 2: | CTTCTCAGGAGCTTTGTGGG |