| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.251883 |
| Chromosome: | chromosome 8 |
| Location: | 4725915 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCTGGCAGTGGTGGAGGCGGGCGGGCCA |
| Internal bar code: | GAGTCCTATACCCGCACCGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 527 |
| LEAP-Seq percent confirming: | 78.5216 |
| LEAP-Seq n confirming: | 563 |
| LEAP-Seq n nonconfirming: | 154 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCTTGCACTTGCCTTGT |
| Suggested primer 2: | AATTTGTGCTCCTCGCATCT |