| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.251885 |
| Chromosome: | chromosome 5 |
| Location: | 2725481 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236802 | (1 of 71) IPR016181 - Acyl-CoA N-acyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCACACAGAGGGCACACGAACGGGGAT |
| Internal bar code: | CCTCGTGGGCAAGGCACTCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 583 |
| LEAP-Seq percent confirming: | 95.2869 |
| LEAP-Seq n confirming: | 465 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCACTACTCCTGCTGGT |
| Suggested primer 2: | CTACGGAAAGATTGCGCTTC |