| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251886 |
| Chromosome: | chromosome 2 |
| Location: | 3537241 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095127 | (1 of 3) PTHR11732//PTHR11732:SF146 - ALDO/KETO REDUCTASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCATGCGCCCTTCCCTCATCCCCGTCC |
| Internal bar code: | CGGGATGAAGGTTGTCATTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 21 |
| LEAP-Seq percent confirming: | 99.6205 |
| LEAP-Seq n confirming: | 525 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTCGTAGGTAGGGGACA |
| Suggested primer 2: | ACCATCTATGCGGTGGTCTC |