| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251896 |
| Chromosome: | chromosome 15 |
| Location: | 224373 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635400 | ZYS3,ZYS3-1 | (1 of 3) PTHR24193//PTHR24193:SF87 - ANKYRIN REPEAT PROTEIN // SUBFAMILY NOT NAMED; Zygote-specific protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAACAGCTTATTTGCAGGAGCGCCAGC |
| Internal bar code: | GGGTGGAGGGTCTGGCGTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 151 |
| LEAP-Seq percent confirming: | 99.7506 |
| LEAP-Seq n confirming: | 400 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCACTGCCACGATACCTC |
| Suggested primer 2: | ATGAGGGAGGCATGGTGTAG |