| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251906 |
| Chromosome: | chromosome_14 |
| Location: | 1579726 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g618750 | FAP246 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGAAATATTGGGAACAGGATACTGGGAAT |
| Internal bar code: | CTGCCTCTTCGACCCCCCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 96.6173 |
| LEAP-Seq n confirming: | 457 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTCCTCAGGTGTGGT |
| Suggested primer 2: | CTCAAGGGAACAGAAGCAGG |