| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.251910 |
| Chromosome: | chromosome 10 |
| Location: | 6146866 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463900 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAACAGTCGGGGGGGCGGTACAGGGCGC |
| Internal bar code: | TCAGGACGTGGGGCGCATGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 155 |
| LEAP-Seq percent confirming: | 97.7528 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAGTGTGTGTGCCGATGT |
| Suggested primer 2: | TGGCTGGGTACTCAGGGTAG |