| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.251926 |
| Chromosome: | chromosome 7 |
| Location: | 5149371 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348200 | SPH3 | Sucrose-phosphate phosphatase; (1 of 1) PTHR12526:SF2 - SUCROSE-PHOSPHATASE 1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAATGAGGCTACAGCCGCCGCCGCCAGCA |
| Internal bar code: | ACTCAGGCTCGCTCCAGGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 441 |
| LEAP-Seq percent confirming: | 94.7917 |
| LEAP-Seq n confirming: | 182 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCCTGAGCTTTGGACCTG |
| Suggested primer 2: | ACACCAGATCAACACGTCCA |