| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.251964 |
| Chromosome: | chromosome 12 |
| Location: | 2253974 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507950 | CGL98 | possible permease; (1 of 1) PTHR22911//PTHR22911:SF60 - ACYL-MALONYL CONDENSING ENZYME-RELATED // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGCCTTGAACCCTGTTCCCTCTTGTTT |
| Internal bar code: | GGCGCCGTCTATCGTCGCGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 110 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAGCCAACGTTTGAAGAT |
| Suggested primer 2: | CAGACCCGTCCTGTTCGTAT |