| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.252004 |
| Chromosome: | chromosome 13 |
| Location: | 2019965 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576800 | PHX21,HIF1,HIF | (1 of 1) K09592 - hypoxia-inducible factor prolyl hydroxylase (EGLN, HPH); Cytosolic prolyl 4-hydroxylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGAAACAGCTGCTGCGCTAGCGTCTCCA |
| Internal bar code: | CTTACTAAGAGGTCGCTCATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 526 |
| LEAP-Seq percent confirming: | 97.931 |
| LEAP-Seq n confirming: | 710 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGAATCGAGACCGATGG |
| Suggested primer 2: | TCTTACCACCCCTTGTGTCC |