| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.252073 |
| Chromosome: | chromosome 13 |
| Location: | 1616798 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573500 | BUG12,FAP95 | Flagellar Associated Protein 95 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGTTCTTCGCGAGTGCGGCATTCAGCT |
| Internal bar code: | ATGTAACGTCGGGCGTGAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 523 |
| LEAP-Seq percent confirming: | 96.7391 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATCTGCATGTGCTTACA |
| Suggested primer 2: | CACCACTCCGACGGTAACTT |