| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.252081 |
| Chromosome: | chromosome_9 |
| Location: | 3324481 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g388393 | ELG13 | Exostosin-like glycosyltransferase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGAGATAGGTACCCACACACTTCGTGTCT |
| Internal bar code: | GTCAACTTTAAAGTGCCGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 809 |
| LEAP-Seq percent confirming: | 99.6056 |
| LEAP-Seq n confirming: | 6818 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAACGATGCAAAGCACTC |
| Suggested primer 2: | TGAGTACCAGCACCTCAACG |