Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.252094 |
Chromosome: | chromosome_8 |
Location: | 3412685 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g374700 | FAP244 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGACAGCGCTCACAGGGACGAGCTAGCGCT |
Internal bar code: | ATCAAGACGTTTCAGCAACACT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 544 |
LEAP-Seq percent confirming: | 99.1525 |
LEAP-Seq n confirming: | 117 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCATGCTGCTTTCTAGTCC |
Suggested primer 2: | AGTCGCTGTCAGGTTGTCCT |