Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.252094 |
Chromosome: | chromosome_12 |
Location: | 5903379 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g534050 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCCCCCGTTCGCGCTCAGGTCCTCTGCTG |
Internal bar code: | CACTTGCACGAGAGGCCATTCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 301 |
LEAP-Seq percent confirming: | 98.6111 |
LEAP-Seq n confirming: | 71 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCGTAGAAGGAGGAGCATT |
Suggested primer 2: | ACTACCCGTACGAGTGGACG |