| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.252148 |
| Chromosome: | chromosome 9 |
| Location: | 5782452 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401997 | (1 of 1) 1.1.1.60 - 2-hydroxy-3-oxopropionate reductase / Tartronate semialdehyde reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTGGCACCGCGCCCGCCGCCCCAGCCCC |
| Internal bar code: | AGGAGGTACGTGGATGTAGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 197 |
| LEAP-Seq percent confirming: | 15.3846 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGACAACAATTTCGGCAT |
| Suggested primer 2: | AGCAAATGGGCTATGGAGTG |