| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.252150 |
| Chromosome: | chromosome 9 |
| Location: | 2205721 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393000 | CSR6 | (1 of 6) 2.8.2.8 - [Heparan sulfate]-glucosamine N-sulfotransferase / PAPS:N-desulfoheparin sulfotransferase; Carbohydrate sulfotransferase-related 6 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAATGATGCGGCGCCGGGCTAGTGCGGTT |
| Internal bar code: | ATGGTATCAAATTTTTTTCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 512 |
| LEAP-Seq percent confirming: | 99.0685 |
| LEAP-Seq n confirming: | 5424 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGGAAGACGATGTTGTT |
| Suggested primer 2: | AGGGCCAACATACAAGAACG |