Insertion junction: LMJ.RY0402.252171_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.252171
Chromosome:chromosome 2
Location:6918436
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g119850 PDE9 (1 of 3) 3.1.4.17//3.1.4.53 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase // 3',5'-cyclic-AMP phosphodiesterase / cAMP-specific phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGAATCACAGGCGGACTGGTGAGTAATA
Internal bar code:GGGATGGGATGATCCCTGCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:611
LEAP-Seq percent confirming:99.3631
LEAP-Seq n confirming:156
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTTCCCGTCACCACATAC
Suggested primer 2:TGTGCTGGAGGAGGAGTTCT