| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.252178 |
| Chromosome: | chromosome 14 |
| Location: | 3687225 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631650 | FAP406 | Flagellar Associated protein 406 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCTGGTGGCTTCCCCTCCCCCTCGACC |
| Internal bar code: | AGGTCCAAGTTGAGGTACGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 365 |
| LEAP-Seq percent confirming: | 98.4528 |
| LEAP-Seq n confirming: | 1209 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTCCCCTATTCCCCATCT |
| Suggested primer 2: | CCACCTGTACGGCGAATACT |