Insertion junction: LMJ.RY0402.252211_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.252211
Chromosome:chromosome 7
Location:5798858
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g353200 (1 of 1) IPR006073//IPR016496//IPR025121//IPR027417//IPR030394 - GTP binding domain // GTPase HflX // GTPase HflX, N-terminal // P-loop containing nucleoside triphosphate hydrolase // HflX-type guanine nucleotide-binding (G) domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGAGGAGCTGATTCGGTTCCTGAGGAGG
Internal bar code:AGGGGCCACGTCGTCTTCCCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:410
LEAP-Seq percent confirming:96.3855
LEAP-Seq n confirming:160
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TTCACCTAGCCTCTGGCTGT
Suggested primer 2:AGCGCTTGCTCAATTACCAT