Insertion junction: LMJ.RY0402.252216_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.252216
Chromosome:chromosome 10
Location:5199959
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g456900 FPN4 (1 of 2) PTHR12103//PTHR12103:SF21 - CYTOSOLIC PURINE 5-NUCLEOTIDASE-RELATED // IMP-GMP SPECIFIC 5-NUCLEOTIDASE, PUTATIVE-RELATED; Purine 5'-nucleotidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACGACGACCGCATCCAGCGCCTCAAGTG
Internal bar code:GTTTCGGGACGCTCTGCACGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:585
LEAP-Seq percent confirming:25.6626
LEAP-Seq n confirming:3379
LEAP-Seq n nonconfirming:9788
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:TACATCGGCGACCACATCTA
Suggested primer 2:ATCTGGCAGAATAACCGTGG