| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.252216 |
| Chromosome: | chromosome_10 |
| Location: | 5199959 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g456900 | FPN4 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCATCCCGCAGCTGGCGCAGCTGCCGCAGC |
| Internal bar code: | TGAATTCCTCGCTCTTGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 99.0415 |
| LEAP-Seq n confirming: | 930 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACATCGGCGACCACATCTA |
| Suggested primer 2: | ATCTGGCAGAATAACCGTGG |