| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.252221 |
| Chromosome: | chromosome_1 |
| Location: | 3654649 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g023550 | FAP234 | Flagellar Associated Protein, putative outer arm dynein light ch | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCATGGGGGCCGGGGCCGCAGGGCGGCGT |
| Internal bar code: | TAAGGCGAACGAGGGGTGTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 115 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCTTGTCCAACTCGGTGT |
| Suggested primer 2: | ATCAAGGCTGTCCAGAATGG |