Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.252273 |
Chromosome: | chromosome 14 |
Location: | 3684015 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCACACGAGGGGGAGGGCGTGGGGGGAG |
Internal bar code: | CACAGCCAGCGATGGTCCAAAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 513 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 376 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCACCTTGACTTGGTGACCT |
Suggested primer 2: | AGTAATCAAACGCCACTGGG |