Insertion junction: LMJ.RY0402.252273_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.252273
Chromosome:chromosome 14
Location:3684015
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCACACGAGGGGGAGGGCGTGGGGGGAG
Internal bar code:CACAGCCAGCGATGGTCCAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:513
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:376
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCTTGACTTGGTGACCT
Suggested primer 2:AGTAATCAAACGCCACTGGG