Insertion junction: LMJ.RY0402.252275_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.252275
Chromosome:chromosome 3
Location:9165564
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g212641 (1 of 16) 2.3.1.48 - Histone acetyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGCCTCCCGGAGACGCTCGCTCTCATTC
Internal bar code:TAAGCTCAGCATCCGGTCTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:578
LEAP-Seq percent confirming:97.9675
LEAP-Seq n confirming:241
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCGGGTTGTGCAACTTAT
Suggested primer 2:AACCGTGGTTTTGTCTGAGG