Insertion junction: LMJ.RY0402.252275_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.252275
Chromosome:chromosome 11
Location:2741937
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g477100 FAP97 (1 of 1) IPR003882//IPR029488 - Pistil-specific extensin-like protein // KIAA1430 homologue; Flagellar Associated Protein 97 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAGAACACCTTAACGATTACATTATCGC
Internal bar code:ACGCACTAGGGGACGCGAGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:470
LEAP-Seq percent confirming:99.3878
LEAP-Seq n confirming:487
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGCAACTCGGTCAATTTT
Suggested primer 2:CAGCACTCCGCTTTGTATGA