| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.252279 |
| Chromosome: | chromosome 9 |
| Location: | 1738159 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396650 | PAT2 | Phosphate acetyltransferase; (1 of 2) 2.3.1.8 - Phosphate acetyltransferase / Phosphotransacetylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTACGCCCCGCCTCACCCTGCAGATCG |
| Internal bar code: | GCTGTTCGGGAGCCCTAGACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 189 |
| LEAP-Seq percent confirming: | 99.8174 |
| LEAP-Seq n confirming: | 1640 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCGAAATAACCCTCTCC |
| Suggested primer 2: | ACCCTCTTCCTCTCGGACAT |